University of Buea
Onchocerciasis LAMP assay to optimize diagnostics and sampling strategies for low-prevalence settings in Cameroon
1. Can the O-150 LAMP assay be established on whole blood? 2. Can the O-150 qPCR methodology be adapted for human sampling?
1. How does the performance of the O-150 LAMP compare to the O-150 qPCR assay?
2. What threshold be used to define positive vs negative results for both assays?
3. Can deep sequencing reveal over potential biomarkers for further investigation?
Through funding from the Wellcome Trust to develop a global atlas of podoconiosis. We aim to advance new knowledge on the geographical distribution and spatial epidemiology of the disease.
i. Conduct national cross-sectional surveys in selected countries to validate the environmental predictive model developed using the mapping data in Ethiopia.
ii. Create evidence consensus maps, develop risk maps and ground-truthing work and delineate the spatial distribution and geographical limits of podoconiosis globally.
iii. Estimate the global burden of podoconiosis by quantifying the number affected, the population at risk and DALYs attributable.
iv. Estimate how much it will cost to control or eliminate podoconiosis globally.