Laboratory analysis of Ov16 ELISA and Skin snip PCR to support surveillance activities in National programs. Multi-country comparison of diagnostic tools to detect Onchocerca volvulus.
To compare the performance of the diagnostic tools currently available for O. volvulus in terms of their relative sensitivity, species-specificity and practical use by countries. Comparison of the utility of these tools for mapping and surveillance in settings with different levels of endemicity for onchocerciasis (Oncho), lymphatic filariasis (LF) and/or loiasis.
To determine if there is evidence of ongoing transmission of lymphatic filariasis in Mindoro Oriental, following a TAS 2 failure.
To assess the relationships between the prevalence of the clinical sign TF compared to prevalence of infection and antibody in Chikwawa and Mchinji districts
To determine if a standardized multi-parallel-PCR assay is a more sensitive diagnostic tool for detecting Hookworm, Trichuris trichiura, Ascaris lumbricoides, and Strongyloides prevalence compared to the Kato-Katz stool test.
Understanding the best uses of the Supervisor's Coverage Tool (SCT) for monitoring school-based distributions
- To use the Supervisor's Coverage Tool (SCT) to monitor school-based deworming;
- To determine the feasibility of utilizing the Lot Quality Assurance Sampling (LQAS) methodology in a school-based SCT; and
- To apply a checklist in schools to elicit information about the performance of the MDA.
To study the feasibility of LF and Oncho (Filariases) integrated transmission assessment survey (F-TAS) according to both LF and Onchocerciasis WHO elimination guidelines
1) To analyze the ELISA (Wb123) results from the field sampling
2) To conduct the mosquito field collection
3) To analyze the mosquito samples using PCR
Primary Objective: To test the 4,500 samples collected as part of the TAS Strengthening Study in Tanzania for Wb123 and Ov16 antibodies using ELISA.
Secondary Objective: To build capacity at the Tanga laboratory so that it can serve as a regional lab for future specimen processing.
The TAS Strengthening Study in Tanzania (101G) is designed to assess additional indicators that may be added to the current TAS platform in order to strengthen the resulting stopping or surveillance decisions. The ELISA results will be merged with the antigen and demographic data, and ultimately with the xenomonitoring data. A comprehensive analysis will be conducted to understand the correlation between antigen and antibody in adults and children with the mosquito data. A spatial analysis looking at microfoci of infection will also be conducted. The ELISA results will feed into a larger multi-country analysis. The results are expected to help inform WHO guidance on stopping and surveillance for LF.